Synthesized:
Reverse Transcription:
Article Title: Selective control of primer usage in multiplex one-step reverse transcription PCR
Article Snippet: .. Reaction conditions included 1× PCR buffer (20 mM Tris (pH 8.4), 50 mM KCl) (Invitrogen), 1.5 mM MgCl 2 (Invitrogen), gene-specific PCR primers (0.5 μM) (TriLink), oligo(dT) 18 primer (1 μM) (TriLink), 0.16 mM dNTPs (New England Biolabs), 0.5 μL of human trachea total RNA or other human total RNA (Stratagene or Ambion, each at ~1.6 μg/μL), 5 U RNase Inhibitor (New England Biolabs), 50 U Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) (Invitrogen) or SuperScript III Reverse Transcriptase (SSIII RT) (Invitrogen), and 0.6 U of Taq DNA Polymerase, recombinant (Invitrogen) or Platinum Taq DNA Polymerase (Invitrogen) or AmpliTaq Gold DNA Polymerase (Applied Biosystems), in a 50 μL reaction volume. ..
Article Title: Multifaceted contributions of Dicer2 to arbovirus transmission by Aedes aegypti.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Dengue Virus Complex Antibody, clone D3-2H2-9-21 Merck-Milipore MAB8705; RRID: AB_95338 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Life Technologies A-11029; RRID: AB_2534088 Bacterial and virus strains DENV-1 KDH0026A Ref. #56 GenBank: HG316481 DENV-3 GA28-7 Ref. #57 GenBank: JX080299.1 ZIKV FSS13025 Ref. #58 N/A CHIKV M105 Ref. #59 GenBank: LN898104.1 MAYV TRVL 4675 Ref. #60 N/A CFAV-KPP Ref. #61 ENA: LR596014 Chemicals, peptides, and recombinant proteins Cas9 Nuclease, S. pyogenes New England Biolabs Cat#M0646 Critical commercial assays DNAzol DIRECT Molecular Research Center, Inc. Cat#DN131 TRIzol Reagent Thermo Fisher Scientific Cat#15596026 DreamTaq Green DNA Polymerase Thermo Fisher Scientific Cat#EP0714 MEGAscript T7 in vitro transcription kit Life Technologies Cat#AM1333 M-MLV Reverse Transcriptase Thermo Fisher Scientific Cat28025013 RNaseOUT Recombinant Ribonuclease Inhibitor Thermo Fisher Scientific Cat#10777019 GoTaq Probe 1-Step RT-qPCR System Promega Cat#A6002 MEGAscript RNAi kit Life Technologies Cat#AM1626M NEBNext Multiplex Small RNA Library Prep Set for Illumina New England Biolabs Cat#E7300L Universal miRNA Cloning Linker New England Biolabs Cat#S1315S Dual-Luciferase Reporter Assay System Promega Cat#E1910 MinElute PCR Purification Kit Qiagen Cat#2552893 Cellfectin II Thermo Fisher Scientific Cat#10362100 DNAseI Thermo Fisher Scientific Cat#AM1906 GoTaq 1-Step RT-qPCR System Promega Cat#A6020 Deposited data Small RNA sequencing datasets This paper SRA: PRJNA907782 Experimental models: Cell lines Aedes albopictus C6/36 ATCC CRL-1660 Vero E6 ATCC CRL-1586 Experimental models: Organisms/strains Aedes aegypti outbred colony from Bakoumba Ref. #31 N/A Aedes aegypti Dicer2 ( / ) mutant line derived from outbred colony This study N/A Aedes aegypti Dicer2 (+/+) sister line derived from outbred colony This study N/A Oligonucleotides All oligonucleotide sequences are listed in Table S1 N/A Recombinant DNA pUb-GL3 plasmid Ref. #15 N/A (Continued on next page) 16 Cell Reports 42, 112977, August 29, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER pCMV-Rluc plasmid Ref. #15 N/A Software and algorithms FastQC http://www.bioinformatics. babraham.ac.uk/projects/ fastqc/ N/A Cutadapt Ref. #68 N/A Bowtie1 Ref. #69 N/A Rsamtools v1.20.4 (R package) Ref. #71 N/A ShortRead v1.26.0 (R package) Ref. #72 N/A Graphpad Prism v.9.3.1 www.graphpad.com N/A JMP v14.0 www.jmp.com N/A CRISPOR Ref. #62 N/A
Article Title: miRNA-34b as a tumor suppressor in estrogen-dependent growth of breast cancer cells
Article Snippet: The number of colonies was counted after they were stained with 0.05% crystal violet (Sigma-Aldrich) for 1 hour and washed extensively with PBS. .. The human miRNA TLDA was used in conjunction with Multiplex reverse transcriptase (RT; Applied Biosystems) consisting of eight predefined RT primer pools containing up to 48 RT primers each. ..
Article Title: Circulating microRNAs in seminal plasma as predictors of sperm retrieval in microdissection testicular sperm extraction
Article Snippet: .. Multiplex RT-qPCR for microRNAs Briefly, 1 μL of 50 nM SLP cocktail (13 SLP mixture) together with 10 mM dNTPs, 0.1 M DTT, 40 units of RNaseOUTTM (Thermo Fisher; #10777-019) and 200 units of SuperScriptTM II Reverse Transcriptase (SS II RTase) (Thermo Fisher; #18064014) were used for each RT reaction. ..
Multiplex Assay:
Article Title: Multifaceted contributions of Dicer2 to arbovirus transmission by Aedes aegypti.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Dengue Virus Complex Antibody, clone D3-2H2-9-21 Merck-Milipore MAB8705; RRID: AB_95338 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Life Technologies A-11029; RRID: AB_2534088 Bacterial and virus strains DENV-1 KDH0026A Ref. #56 GenBank: HG316481 DENV-3 GA28-7 Ref. #57 GenBank: JX080299.1 ZIKV FSS13025 Ref. #58 N/A CHIKV M105 Ref. #59 GenBank: LN898104.1 MAYV TRVL 4675 Ref. #60 N/A CFAV-KPP Ref. #61 ENA: LR596014 Chemicals, peptides, and recombinant proteins Cas9 Nuclease, S. pyogenes New England Biolabs Cat#M0646 Critical commercial assays DNAzol DIRECT Molecular Research Center, Inc. Cat#DN131 TRIzol Reagent Thermo Fisher Scientific Cat#15596026 DreamTaq Green DNA Polymerase Thermo Fisher Scientific Cat#EP0714 MEGAscript T7 in vitro transcription kit Life Technologies Cat#AM1333 M-MLV Reverse Transcriptase Thermo Fisher Scientific Cat28025013 RNaseOUT Recombinant Ribonuclease Inhibitor Thermo Fisher Scientific Cat#10777019 GoTaq Probe 1-Step RT-qPCR System Promega Cat#A6002 MEGAscript RNAi kit Life Technologies Cat#AM1626M NEBNext Multiplex Small RNA Library Prep Set for Illumina New England Biolabs Cat#E7300L Universal miRNA Cloning Linker New England Biolabs Cat#S1315S Dual-Luciferase Reporter Assay System Promega Cat#E1910 MinElute PCR Purification Kit Qiagen Cat#2552893 Cellfectin II Thermo Fisher Scientific Cat#10362100 DNAseI Thermo Fisher Scientific Cat#AM1906 GoTaq 1-Step RT-qPCR System Promega Cat#A6020 Deposited data Small RNA sequencing datasets This paper SRA: PRJNA907782 Experimental models: Cell lines Aedes albopictus C6/36 ATCC CRL-1660 Vero E6 ATCC CRL-1586 Experimental models: Organisms/strains Aedes aegypti outbred colony from Bakoumba Ref. #31 N/A Aedes aegypti Dicer2 ( / ) mutant line derived from outbred colony This study N/A Aedes aegypti Dicer2 (+/+) sister line derived from outbred colony This study N/A Oligonucleotides All oligonucleotide sequences are listed in Table S1 N/A Recombinant DNA pUb-GL3 plasmid Ref. #15 N/A (Continued on next page) 16 Cell Reports 42, 112977, August 29, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER pCMV-Rluc plasmid Ref. #15 N/A Software and algorithms FastQC http://www.bioinformatics. babraham.ac.uk/projects/ fastqc/ N/A Cutadapt Ref. #68 N/A Bowtie1 Ref. #69 N/A Rsamtools v1.20.4 (R package) Ref. #71 N/A ShortRead v1.26.0 (R package) Ref. #72 N/A Graphpad Prism v.9.3.1 www.graphpad.com N/A JMP v14.0 www.jmp.com N/A CRISPOR Ref. #62 N/A
Article Title: miRNA-34b as a tumor suppressor in estrogen-dependent growth of breast cancer cells
Article Snippet: The number of colonies was counted after they were stained with 0.05% crystal violet (Sigma-Aldrich) for 1 hour and washed extensively with PBS. .. The human miRNA TLDA was used in conjunction with Multiplex reverse transcriptase (RT; Applied Biosystems) consisting of eight predefined RT primer pools containing up to 48 RT primers each. ..
Article Title: Circulating microRNAs in seminal plasma as predictors of sperm retrieval in microdissection testicular sperm extraction
Article Snippet: .. Multiplex RT-qPCR for microRNAs Briefly, 1 μL of 50 nM SLP cocktail (13 SLP mixture) together with 10 mM dNTPs, 0.1 M DTT, 40 units of RNaseOUTTM (Thermo Fisher; #10777-019) and 200 units of SuperScriptTM II Reverse Transcriptase (SS II RTase) (Thermo Fisher; #18064014) were used for each RT reaction. ..
Polymerase Chain Reaction:
Article Title: Selective control of primer usage in multiplex one-step reverse transcription PCR
Article Snippet: .. Reaction conditions included 1× PCR buffer (20 mM Tris (pH 8.4), 50 mM KCl) (Invitrogen), 1.5 mM MgCl 2 (Invitrogen), gene-specific PCR primers (0.5 μM) (TriLink), oligo(dT) 18 primer (1 μM) (TriLink), 0.16 mM dNTPs (New England Biolabs), 0.5 μL of human trachea total RNA or other human total RNA (Stratagene or Ambion, each at ~1.6 μg/μL), 5 U RNase Inhibitor (New England Biolabs), 50 U Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) (Invitrogen) or SuperScript III Reverse Transcriptase (SSIII RT) (Invitrogen), and 0.6 U of Taq DNA Polymerase, recombinant (Invitrogen) or Platinum Taq DNA Polymerase (Invitrogen) or AmpliTaq Gold DNA Polymerase (Applied Biosystems), in a 50 μL reaction volume. ..
Article Title: Multifaceted contributions of Dicer2 to arbovirus transmission by Aedes aegypti.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Dengue Virus Complex Antibody, clone D3-2H2-9-21 Merck-Milipore MAB8705; RRID: AB_95338 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Life Technologies A-11029; RRID: AB_2534088 Bacterial and virus strains DENV-1 KDH0026A Ref. #56 GenBank: HG316481 DENV-3 GA28-7 Ref. #57 GenBank: JX080299.1 ZIKV FSS13025 Ref. #58 N/A CHIKV M105 Ref. #59 GenBank: LN898104.1 MAYV TRVL 4675 Ref. #60 N/A CFAV-KPP Ref. #61 ENA: LR596014 Chemicals, peptides, and recombinant proteins Cas9 Nuclease, S. pyogenes New England Biolabs Cat#M0646 Critical commercial assays DNAzol DIRECT Molecular Research Center, Inc. Cat#DN131 TRIzol Reagent Thermo Fisher Scientific Cat#15596026 DreamTaq Green DNA Polymerase Thermo Fisher Scientific Cat#EP0714 MEGAscript T7 in vitro transcription kit Life Technologies Cat#AM1333 M-MLV Reverse Transcriptase Thermo Fisher Scientific Cat28025013 RNaseOUT Recombinant Ribonuclease Inhibitor Thermo Fisher Scientific Cat#10777019 GoTaq Probe 1-Step RT-qPCR System Promega Cat#A6002 MEGAscript RNAi kit Life Technologies Cat#AM1626M NEBNext Multiplex Small RNA Library Prep Set for Illumina New England Biolabs Cat#E7300L Universal miRNA Cloning Linker New England Biolabs Cat#S1315S Dual-Luciferase Reporter Assay System Promega Cat#E1910 MinElute PCR Purification Kit Qiagen Cat#2552893 Cellfectin II Thermo Fisher Scientific Cat#10362100 DNAseI Thermo Fisher Scientific Cat#AM1906 GoTaq 1-Step RT-qPCR System Promega Cat#A6020 Deposited data Small RNA sequencing datasets This paper SRA: PRJNA907782 Experimental models: Cell lines Aedes albopictus C6/36 ATCC CRL-1660 Vero E6 ATCC CRL-1586 Experimental models: Organisms/strains Aedes aegypti outbred colony from Bakoumba Ref. #31 N/A Aedes aegypti Dicer2 ( / ) mutant line derived from outbred colony This study N/A Aedes aegypti Dicer2 (+/+) sister line derived from outbred colony This study N/A Oligonucleotides All oligonucleotide sequences are listed in Table S1 N/A Recombinant DNA pUb-GL3 plasmid Ref. #15 N/A (Continued on next page) 16 Cell Reports 42, 112977, August 29, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER pCMV-Rluc plasmid Ref. #15 N/A Software and algorithms FastQC http://www.bioinformatics. babraham.ac.uk/projects/ fastqc/ N/A Cutadapt Ref. #68 N/A Bowtie1 Ref. #69 N/A Rsamtools v1.20.4 (R package) Ref. #71 N/A ShortRead v1.26.0 (R package) Ref. #72 N/A Graphpad Prism v.9.3.1 www.graphpad.com N/A JMP v14.0 www.jmp.com N/A CRISPOR Ref. #62 N/A
Virus:Article Title: Selective control of primer usage in multiplex one-step reverse transcription PCR
Article Snippet: .. Reaction conditions included 1× PCR buffer (20 mM Tris (pH 8.4), 50 mM KCl) (Invitrogen), 1.5 mM MgCl 2 (Invitrogen), gene-specific PCR primers (0.5 μM) (TriLink), oligo(dT) 18 primer (1 μM) (TriLink), 0.16 mM dNTPs (New England Biolabs), 0.5 μL of human trachea total RNA or other human total RNA (Stratagene or Ambion, each at ~1.6 μg/μL), 5 U RNase Inhibitor (New England Biolabs), 50 U Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) (Invitrogen) or SuperScript III Reverse Transcriptase (SSIII RT) (Invitrogen), and 0.6 U of Taq DNA Polymerase, recombinant (Invitrogen) or Platinum Taq DNA Polymerase (Invitrogen) or AmpliTaq Gold DNA Polymerase (Applied Biosystems), in a 50 μL reaction volume. ..
Article Title: Multifaceted contributions of Dicer2 to arbovirus transmission by Aedes aegypti.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Dengue Virus Complex Antibody, clone D3-2H2-9-21 Merck-Milipore MAB8705; RRID: AB_95338 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Life Technologies A-11029; RRID: AB_2534088 Bacterial and virus strains DENV-1 KDH0026A Ref. #56 GenBank: HG316481 DENV-3 GA28-7 Ref. #57 GenBank: JX080299.1 ZIKV FSS13025 Ref. #58 N/A CHIKV M105 Ref. #59 GenBank: LN898104.1 MAYV TRVL 4675 Ref. #60 N/A CFAV-KPP Ref. #61 ENA: LR596014 Chemicals, peptides, and recombinant proteins Cas9 Nuclease, S. pyogenes New England Biolabs Cat#M0646 Critical commercial assays DNAzol DIRECT Molecular Research Center, Inc. Cat#DN131 TRIzol Reagent Thermo Fisher Scientific Cat#15596026 DreamTaq Green DNA Polymerase Thermo Fisher Scientific Cat#EP0714 MEGAscript T7 in vitro transcription kit Life Technologies Cat#AM1333 M-MLV Reverse Transcriptase Thermo Fisher Scientific Cat28025013 RNaseOUT Recombinant Ribonuclease Inhibitor Thermo Fisher Scientific Cat#10777019 GoTaq Probe 1-Step RT-qPCR System Promega Cat#A6002 MEGAscript RNAi kit Life Technologies Cat#AM1626M NEBNext Multiplex Small RNA Library Prep Set for Illumina New England Biolabs Cat#E7300L Universal miRNA Cloning Linker New England Biolabs Cat#S1315S Dual-Luciferase Reporter Assay System Promega Cat#E1910 MinElute PCR Purification Kit Qiagen Cat#2552893 Cellfectin II Thermo Fisher Scientific Cat#10362100 DNAseI Thermo Fisher Scientific Cat#AM1906 GoTaq 1-Step RT-qPCR System Promega Cat#A6020 Deposited data Small RNA sequencing datasets This paper SRA: PRJNA907782 Experimental models: Cell lines Aedes albopictus C6/36 ATCC CRL-1660 Vero E6 ATCC CRL-1586 Experimental models: Organisms/strains Aedes aegypti outbred colony from Bakoumba Ref. #31 N/A Aedes aegypti Dicer2 ( / ) mutant line derived from outbred colony This study N/A Aedes aegypti Dicer2 (+/+) sister line derived from outbred colony This study N/A Oligonucleotides All oligonucleotide sequences are listed in Table S1 N/A Recombinant DNA pUb-GL3 plasmid Ref. #15 N/A (Continued on next page) 16 Cell Reports 42, 112977, August 29, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER pCMV-Rluc plasmid Ref. #15 N/A Software and algorithms FastQC http://www.bioinformatics. babraham.ac.uk/projects/ fastqc/ N/A Cutadapt Ref. #68 N/A Bowtie1 Ref. #69 N/A Rsamtools v1.20.4 (R package) Ref. #71 N/A ShortRead v1.26.0 (R package) Ref. #72 N/A Graphpad Prism v.9.3.1 www.graphpad.com N/A JMP v14.0 www.jmp.com N/A CRISPOR Ref. #62 N/A
Recombinant:Article Title: Selective control of primer usage in multiplex one-step reverse transcription PCR
Article Snippet: .. Reaction conditions included 1× PCR buffer (20 mM Tris (pH 8.4), 50 mM KCl) (Invitrogen), 1.5 mM MgCl 2 (Invitrogen), gene-specific PCR primers (0.5 μM) (TriLink), oligo(dT) 18 primer (1 μM) (TriLink), 0.16 mM dNTPs (New England Biolabs), 0.5 μL of human trachea total RNA or other human total RNA (Stratagene or Ambion, each at ~1.6 μg/μL), 5 U RNase Inhibitor (New England Biolabs), 50 U Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) (Invitrogen) or SuperScript III Reverse Transcriptase (SSIII RT) (Invitrogen), and 0.6 U of Taq DNA Polymerase, recombinant (Invitrogen) or Platinum Taq DNA Polymerase (Invitrogen) or AmpliTaq Gold DNA Polymerase (Applied Biosystems), in a 50 μL reaction volume. ..
Article Title: Multifaceted contributions of Dicer2 to arbovirus transmission by Aedes aegypti.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Dengue Virus Complex Antibody, clone D3-2H2-9-21 Merck-Milipore MAB8705; RRID: AB_95338 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Life Technologies A-11029; RRID: AB_2534088 Bacterial and virus strains DENV-1 KDH0026A Ref. #56 GenBank: HG316481 DENV-3 GA28-7 Ref. #57 GenBank: JX080299.1 ZIKV FSS13025 Ref. #58 N/A CHIKV M105 Ref. #59 GenBank: LN898104.1 MAYV TRVL 4675 Ref. #60 N/A CFAV-KPP Ref. #61 ENA: LR596014 Chemicals, peptides, and recombinant proteins Cas9 Nuclease, S. pyogenes New England Biolabs Cat#M0646 Critical commercial assays DNAzol DIRECT Molecular Research Center, Inc. Cat#DN131 TRIzol Reagent Thermo Fisher Scientific Cat#15596026 DreamTaq Green DNA Polymerase Thermo Fisher Scientific Cat#EP0714 MEGAscript T7 in vitro transcription kit Life Technologies Cat#AM1333 M-MLV Reverse Transcriptase Thermo Fisher Scientific Cat28025013 RNaseOUT Recombinant Ribonuclease Inhibitor Thermo Fisher Scientific Cat#10777019 GoTaq Probe 1-Step RT-qPCR System Promega Cat#A6002 MEGAscript RNAi kit Life Technologies Cat#AM1626M NEBNext Multiplex Small RNA Library Prep Set for Illumina New England Biolabs Cat#E7300L Universal miRNA Cloning Linker New England Biolabs Cat#S1315S Dual-Luciferase Reporter Assay System Promega Cat#E1910 MinElute PCR Purification Kit Qiagen Cat#2552893 Cellfectin II Thermo Fisher Scientific Cat#10362100 DNAseI Thermo Fisher Scientific Cat#AM1906 GoTaq 1-Step RT-qPCR System Promega Cat#A6020 Deposited data Small RNA sequencing datasets This paper SRA: PRJNA907782 Experimental models: Cell lines Aedes albopictus C6/36 ATCC CRL-1660 Vero E6 ATCC CRL-1586 Experimental models: Organisms/strains Aedes aegypti outbred colony from Bakoumba Ref. #31 N/A Aedes aegypti Dicer2 ( / ) mutant line derived from outbred colony This study N/A Aedes aegypti Dicer2 (+/+) sister line derived from outbred colony This study N/A Oligonucleotides All oligonucleotide sequences are listed in Table S1 N/A Recombinant DNA pUb-GL3 plasmid Ref. #15 N/A (Continued on next page) 16 Cell Reports 42, 112977, August 29, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER pCMV-Rluc plasmid Ref. #15 N/A Software and algorithms FastQC http://www.bioinformatics. babraham.ac.uk/projects/ fastqc/ N/A Cutadapt Ref. #68 N/A Bowtie1 Ref. #69 N/A Rsamtools v1.20.4 (R package) Ref. #71 N/A ShortRead v1.26.0 (R package) Ref. #72 N/A Graphpad Prism v.9.3.1 www.graphpad.com N/A JMP v14.0 www.jmp.com N/A CRISPOR Ref. #62 N/A
Platinum:Article Title: Selective control of primer usage in multiplex one-step reverse transcription PCR
Article Snippet: .. Reaction conditions included 1× PCR buffer (20 mM Tris (pH 8.4), 50 mM KCl) (Invitrogen), 1.5 mM MgCl 2 (Invitrogen), gene-specific PCR primers (0.5 μM) (TriLink), oligo(dT) 18 primer (1 μM) (TriLink), 0.16 mM dNTPs (New England Biolabs), 0.5 μL of human trachea total RNA or other human total RNA (Stratagene or Ambion, each at ~1.6 μg/μL), 5 U RNase Inhibitor (New England Biolabs), 50 U Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) (Invitrogen) or SuperScript III Reverse Transcriptase (SSIII RT) (Invitrogen), and 0.6 U of Taq DNA Polymerase, recombinant (Invitrogen) or Platinum Taq DNA Polymerase (Invitrogen) or AmpliTaq Gold DNA Polymerase (Applied Biosystems), in a 50 μL reaction volume. ..
In Vitro:Article Title: Multifaceted contributions of Dicer2 to arbovirus transmission by Aedes aegypti.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Dengue Virus Complex Antibody, clone D3-2H2-9-21 Merck-Milipore MAB8705; RRID: AB_95338 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Life Technologies A-11029; RRID: AB_2534088 Bacterial and virus strains DENV-1 KDH0026A Ref. #56 GenBank: HG316481 DENV-3 GA28-7 Ref. #57 GenBank: JX080299.1 ZIKV FSS13025 Ref. #58 N/A CHIKV M105 Ref. #59 GenBank: LN898104.1 MAYV TRVL 4675 Ref. #60 N/A CFAV-KPP Ref. #61 ENA: LR596014 Chemicals, peptides, and recombinant proteins Cas9 Nuclease, S. pyogenes New England Biolabs Cat#M0646 Critical commercial assays DNAzol DIRECT Molecular Research Center, Inc. Cat#DN131 TRIzol Reagent Thermo Fisher Scientific Cat#15596026 DreamTaq Green DNA Polymerase Thermo Fisher Scientific Cat#EP0714 MEGAscript T7 in vitro transcription kit Life Technologies Cat#AM1333 M-MLV Reverse Transcriptase Thermo Fisher Scientific Cat28025013 RNaseOUT Recombinant Ribonuclease Inhibitor Thermo Fisher Scientific Cat#10777019 GoTaq Probe 1-Step RT-qPCR System Promega Cat#A6002 MEGAscript RNAi kit Life Technologies Cat#AM1626M NEBNext Multiplex Small RNA Library Prep Set for Illumina New England Biolabs Cat#E7300L Universal miRNA Cloning Linker New England Biolabs Cat#S1315S Dual-Luciferase Reporter Assay System Promega Cat#E1910 MinElute PCR Purification Kit Qiagen Cat#2552893 Cellfectin II Thermo Fisher Scientific Cat#10362100 DNAseI Thermo Fisher Scientific Cat#AM1906 GoTaq 1-Step RT-qPCR System Promega Cat#A6020 Deposited data Small RNA sequencing datasets This paper SRA: PRJNA907782 Experimental models: Cell lines Aedes albopictus C6/36 ATCC CRL-1660 Vero E6 ATCC CRL-1586 Experimental models: Organisms/strains Aedes aegypti outbred colony from Bakoumba Ref. #31 N/A Aedes aegypti Dicer2 ( / ) mutant line derived from outbred colony This study N/A Aedes aegypti Dicer2 (+/+) sister line derived from outbred colony This study N/A Oligonucleotides All oligonucleotide sequences are listed in Table S1 N/A Recombinant DNA pUb-GL3 plasmid Ref. #15 N/A (Continued on next page) 16 Cell Reports 42, 112977, August 29, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER pCMV-Rluc plasmid Ref. #15 N/A Software and algorithms FastQC http://www.bioinformatics. babraham.ac.uk/projects/ fastqc/ N/A Cutadapt Ref. #68 N/A Bowtie1 Ref. #69 N/A Rsamtools v1.20.4 (R package) Ref. #71 N/A ShortRead v1.26.0 (R package) Ref. #72 N/A Graphpad Prism v.9.3.1 www.graphpad.com N/A JMP v14.0 www.jmp.com N/A CRISPOR Ref. #62 N/A
Quantitative RT-PCR:Article Title: Multifaceted contributions of Dicer2 to arbovirus transmission by Aedes aegypti.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Dengue Virus Complex Antibody, clone D3-2H2-9-21 Merck-Milipore MAB8705; RRID: AB_95338 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Life Technologies A-11029; RRID: AB_2534088 Bacterial and virus strains DENV-1 KDH0026A Ref. #56 GenBank: HG316481 DENV-3 GA28-7 Ref. #57 GenBank: JX080299.1 ZIKV FSS13025 Ref. #58 N/A CHIKV M105 Ref. #59 GenBank: LN898104.1 MAYV TRVL 4675 Ref. #60 N/A CFAV-KPP Ref. #61 ENA: LR596014 Chemicals, peptides, and recombinant proteins Cas9 Nuclease, S. pyogenes New England Biolabs Cat#M0646 Critical commercial assays DNAzol DIRECT Molecular Research Center, Inc. Cat#DN131 TRIzol Reagent Thermo Fisher Scientific Cat#15596026 DreamTaq Green DNA Polymerase Thermo Fisher Scientific Cat#EP0714 MEGAscript T7 in vitro transcription kit Life Technologies Cat#AM1333 M-MLV Reverse Transcriptase Thermo Fisher Scientific Cat28025013 RNaseOUT Recombinant Ribonuclease Inhibitor Thermo Fisher Scientific Cat#10777019 GoTaq Probe 1-Step RT-qPCR System Promega Cat#A6002 MEGAscript RNAi kit Life Technologies Cat#AM1626M NEBNext Multiplex Small RNA Library Prep Set for Illumina New England Biolabs Cat#E7300L Universal miRNA Cloning Linker New England Biolabs Cat#S1315S Dual-Luciferase Reporter Assay System Promega Cat#E1910 MinElute PCR Purification Kit Qiagen Cat#2552893 Cellfectin II Thermo Fisher Scientific Cat#10362100 DNAseI Thermo Fisher Scientific Cat#AM1906 GoTaq 1-Step RT-qPCR System Promega Cat#A6020 Deposited data Small RNA sequencing datasets This paper SRA: PRJNA907782 Experimental models: Cell lines Aedes albopictus C6/36 ATCC CRL-1660 Vero E6 ATCC CRL-1586 Experimental models: Organisms/strains Aedes aegypti outbred colony from Bakoumba Ref. #31 N/A Aedes aegypti Dicer2 ( / ) mutant line derived from outbred colony This study N/A Aedes aegypti Dicer2 (+/+) sister line derived from outbred colony This study N/A Oligonucleotides All oligonucleotide sequences are listed in Table S1 N/A Recombinant DNA pUb-GL3 plasmid Ref. #15 N/A (Continued on next page) 16 Cell Reports 42, 112977, August 29, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER pCMV-Rluc plasmid Ref. #15 N/A Software and algorithms FastQC http://www.bioinformatics. babraham.ac.uk/projects/ fastqc/ N/A Cutadapt Ref. #68 N/A Bowtie1 Ref. #69 N/A Rsamtools v1.20.4 (R package) Ref. #71 N/A ShortRead v1.26.0 (R package) Ref. #72 N/A Graphpad Prism v.9.3.1 www.graphpad.com N/A JMP v14.0 www.jmp.com N/A CRISPOR Ref. #62 N/A
Article Title: Circulating microRNAs in seminal plasma as predictors of sperm retrieval in microdissection testicular sperm extraction
Article Snippet: .. Multiplex RT-qPCR for microRNAs Briefly, 1 μL of 50 nM SLP cocktail (13 SLP mixture) together with 10 mM dNTPs, 0.1 M DTT, 40 units of RNaseOUTTM (Thermo Fisher; #10777-019) and 200 units of SuperScriptTM II Reverse Transcriptase (SS II RTase) (Thermo Fisher; #18064014) were used for each RT reaction. ..
Cloning:Article Title: Multifaceted contributions of Dicer2 to arbovirus transmission by Aedes aegypti.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Dengue Virus Complex Antibody, clone D3-2H2-9-21 Merck-Milipore MAB8705; RRID: AB_95338 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Life Technologies A-11029; RRID: AB_2534088 Bacterial and virus strains DENV-1 KDH0026A Ref. #56 GenBank: HG316481 DENV-3 GA28-7 Ref. #57 GenBank: JX080299.1 ZIKV FSS13025 Ref. #58 N/A CHIKV M105 Ref. #59 GenBank: LN898104.1 MAYV TRVL 4675 Ref. #60 N/A CFAV-KPP Ref. #61 ENA: LR596014 Chemicals, peptides, and recombinant proteins Cas9 Nuclease, S. pyogenes New England Biolabs Cat#M0646 Critical commercial assays DNAzol DIRECT Molecular Research Center, Inc. Cat#DN131 TRIzol Reagent Thermo Fisher Scientific Cat#15596026 DreamTaq Green DNA Polymerase Thermo Fisher Scientific Cat#EP0714 MEGAscript T7 in vitro transcription kit Life Technologies Cat#AM1333 M-MLV Reverse Transcriptase Thermo Fisher Scientific Cat28025013 RNaseOUT Recombinant Ribonuclease Inhibitor Thermo Fisher Scientific Cat#10777019 GoTaq Probe 1-Step RT-qPCR System Promega Cat#A6002 MEGAscript RNAi kit Life Technologies Cat#AM1626M NEBNext Multiplex Small RNA Library Prep Set for Illumina New England Biolabs Cat#E7300L Universal miRNA Cloning Linker New England Biolabs Cat#S1315S Dual-Luciferase Reporter Assay System Promega Cat#E1910 MinElute PCR Purification Kit Qiagen Cat#2552893 Cellfectin II Thermo Fisher Scientific Cat#10362100 DNAseI Thermo Fisher Scientific Cat#AM1906 GoTaq 1-Step RT-qPCR System Promega Cat#A6020 Deposited data Small RNA sequencing datasets This paper SRA: PRJNA907782 Experimental models: Cell lines Aedes albopictus C6/36 ATCC CRL-1660 Vero E6 ATCC CRL-1586 Experimental models: Organisms/strains Aedes aegypti outbred colony from Bakoumba Ref. #31 N/A Aedes aegypti Dicer2 ( / ) mutant line derived from outbred colony This study N/A Aedes aegypti Dicer2 (+/+) sister line derived from outbred colony This study N/A Oligonucleotides All oligonucleotide sequences are listed in Table S1 N/A Recombinant DNA pUb-GL3 plasmid Ref. #15 N/A (Continued on next page) 16 Cell Reports 42, 112977, August 29, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER pCMV-Rluc plasmid Ref. #15 N/A Software and algorithms FastQC http://www.bioinformatics. babraham.ac.uk/projects/ fastqc/ N/A Cutadapt Ref. #68 N/A Bowtie1 Ref. #69 N/A Rsamtools v1.20.4 (R package) Ref. #71 N/A ShortRead v1.26.0 (R package) Ref. #72 N/A Graphpad Prism v.9.3.1 www.graphpad.com N/A JMP v14.0 www.jmp.com N/A CRISPOR Ref. #62 N/A
Reporter Assay:Article Title: Multifaceted contributions of Dicer2 to arbovirus transmission by Aedes aegypti.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Dengue Virus Complex Antibody, clone D3-2H2-9-21 Merck-Milipore MAB8705; RRID: AB_95338 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Life Technologies A-11029; RRID: AB_2534088 Bacterial and virus strains DENV-1 KDH0026A Ref. #56 GenBank: HG316481 DENV-3 GA28-7 Ref. #57 GenBank: JX080299.1 ZIKV FSS13025 Ref. #58 N/A CHIKV M105 Ref. #59 GenBank: LN898104.1 MAYV TRVL 4675 Ref. #60 N/A CFAV-KPP Ref. #61 ENA: LR596014 Chemicals, peptides, and recombinant proteins Cas9 Nuclease, S. pyogenes New England Biolabs Cat#M0646 Critical commercial assays DNAzol DIRECT Molecular Research Center, Inc. Cat#DN131 TRIzol Reagent Thermo Fisher Scientific Cat#15596026 DreamTaq Green DNA Polymerase Thermo Fisher Scientific Cat#EP0714 MEGAscript T7 in vitro transcription kit Life Technologies Cat#AM1333 M-MLV Reverse Transcriptase Thermo Fisher Scientific Cat28025013 RNaseOUT Recombinant Ribonuclease Inhibitor Thermo Fisher Scientific Cat#10777019 GoTaq Probe 1-Step RT-qPCR System Promega Cat#A6002 MEGAscript RNAi kit Life Technologies Cat#AM1626M NEBNext Multiplex Small RNA Library Prep Set for Illumina New England Biolabs Cat#E7300L Universal miRNA Cloning Linker New England Biolabs Cat#S1315S Dual-Luciferase Reporter Assay System Promega Cat#E1910 MinElute PCR Purification Kit Qiagen Cat#2552893 Cellfectin II Thermo Fisher Scientific Cat#10362100 DNAseI Thermo Fisher Scientific Cat#AM1906 GoTaq 1-Step RT-qPCR System Promega Cat#A6020 Deposited data Small RNA sequencing datasets This paper SRA: PRJNA907782 Experimental models: Cell lines Aedes albopictus C6/36 ATCC CRL-1660 Vero E6 ATCC CRL-1586 Experimental models: Organisms/strains Aedes aegypti outbred colony from Bakoumba Ref. #31 N/A Aedes aegypti Dicer2 ( / ) mutant line derived from outbred colony This study N/A Aedes aegypti Dicer2 (+/+) sister line derived from outbred colony This study N/A Oligonucleotides All oligonucleotide sequences are listed in Table S1 N/A Recombinant DNA pUb-GL3 plasmid Ref. #15 N/A (Continued on next page) 16 Cell Reports 42, 112977, August 29, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER pCMV-Rluc plasmid Ref. #15 N/A Software and algorithms FastQC http://www.bioinformatics. babraham.ac.uk/projects/ fastqc/ N/A Cutadapt Ref. #68 N/A Bowtie1 Ref. #69 N/A Rsamtools v1.20.4 (R package) Ref. #71 N/A ShortRead v1.26.0 (R package) Ref. #72 N/A Graphpad Prism v.9.3.1 www.graphpad.com N/A JMP v14.0 www.jmp.com N/A CRISPOR Ref. #62 N/A
Purification:Article Title: Multifaceted contributions of Dicer2 to arbovirus transmission by Aedes aegypti.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Dengue Virus Complex Antibody, clone D3-2H2-9-21 Merck-Milipore MAB8705; RRID: AB_95338 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Life Technologies A-11029; RRID: AB_2534088 Bacterial and virus strains DENV-1 KDH0026A Ref. #56 GenBank: HG316481 DENV-3 GA28-7 Ref. #57 GenBank: JX080299.1 ZIKV FSS13025 Ref. #58 N/A CHIKV M105 Ref. #59 GenBank: LN898104.1 MAYV TRVL 4675 Ref. #60 N/A CFAV-KPP Ref. #61 ENA: LR596014 Chemicals, peptides, and recombinant proteins Cas9 Nuclease, S. pyogenes New England Biolabs Cat#M0646 Critical commercial assays DNAzol DIRECT Molecular Research Center, Inc. Cat#DN131 TRIzol Reagent Thermo Fisher Scientific Cat#15596026 DreamTaq Green DNA Polymerase Thermo Fisher Scientific Cat#EP0714 MEGAscript T7 in vitro transcription kit Life Technologies Cat#AM1333 M-MLV Reverse Transcriptase Thermo Fisher Scientific Cat28025013 RNaseOUT Recombinant Ribonuclease Inhibitor Thermo Fisher Scientific Cat#10777019 GoTaq Probe 1-Step RT-qPCR System Promega Cat#A6002 MEGAscript RNAi kit Life Technologies Cat#AM1626M NEBNext Multiplex Small RNA Library Prep Set for Illumina New England Biolabs Cat#E7300L Universal miRNA Cloning Linker New England Biolabs Cat#S1315S Dual-Luciferase Reporter Assay System Promega Cat#E1910 MinElute PCR Purification Kit Qiagen Cat#2552893 Cellfectin II Thermo Fisher Scientific Cat#10362100 DNAseI Thermo Fisher Scientific Cat#AM1906 GoTaq 1-Step RT-qPCR System Promega Cat#A6020 Deposited data Small RNA sequencing datasets This paper SRA: PRJNA907782 Experimental models: Cell lines Aedes albopictus C6/36 ATCC CRL-1660 Vero E6 ATCC CRL-1586 Experimental models: Organisms/strains Aedes aegypti outbred colony from Bakoumba Ref. #31 N/A Aedes aegypti Dicer2 ( / ) mutant line derived from outbred colony This study N/A Aedes aegypti Dicer2 (+/+) sister line derived from outbred colony This study N/A Oligonucleotides All oligonucleotide sequences are listed in Table S1 N/A Recombinant DNA pUb-GL3 plasmid Ref. #15 N/A (Continued on next page) 16 Cell Reports 42, 112977, August 29, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER pCMV-Rluc plasmid Ref. #15 N/A Software and algorithms FastQC http://www.bioinformatics. babraham.ac.uk/projects/ fastqc/ N/A Cutadapt Ref. #68 N/A Bowtie1 Ref. #69 N/A Rsamtools v1.20.4 (R package) Ref. #71 N/A ShortRead v1.26.0 (R package) Ref. #72 N/A Graphpad Prism v.9.3.1 www.graphpad.com N/A JMP v14.0 www.jmp.com N/A CRISPOR Ref. #62 N/A
RNA Sequencing:Article Title: Multifaceted contributions of Dicer2 to arbovirus transmission by Aedes aegypti.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Dengue Virus Complex Antibody, clone D3-2H2-9-21 Merck-Milipore MAB8705; RRID: AB_95338 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Life Technologies A-11029; RRID: AB_2534088 Bacterial and virus strains DENV-1 KDH0026A Ref. #56 GenBank: HG316481 DENV-3 GA28-7 Ref. #57 GenBank: JX080299.1 ZIKV FSS13025 Ref. #58 N/A CHIKV M105 Ref. #59 GenBank: LN898104.1 MAYV TRVL 4675 Ref. #60 N/A CFAV-KPP Ref. #61 ENA: LR596014 Chemicals, peptides, and recombinant proteins Cas9 Nuclease, S. pyogenes New England Biolabs Cat#M0646 Critical commercial assays DNAzol DIRECT Molecular Research Center, Inc. Cat#DN131 TRIzol Reagent Thermo Fisher Scientific Cat#15596026 DreamTaq Green DNA Polymerase Thermo Fisher Scientific Cat#EP0714 MEGAscript T7 in vitro transcription kit Life Technologies Cat#AM1333 M-MLV Reverse Transcriptase Thermo Fisher Scientific Cat28025013 RNaseOUT Recombinant Ribonuclease Inhibitor Thermo Fisher Scientific Cat#10777019 GoTaq Probe 1-Step RT-qPCR System Promega Cat#A6002 MEGAscript RNAi kit Life Technologies Cat#AM1626M NEBNext Multiplex Small RNA Library Prep Set for Illumina New England Biolabs Cat#E7300L Universal miRNA Cloning Linker New England Biolabs Cat#S1315S Dual-Luciferase Reporter Assay System Promega Cat#E1910 MinElute PCR Purification Kit Qiagen Cat#2552893 Cellfectin II Thermo Fisher Scientific Cat#10362100 DNAseI Thermo Fisher Scientific Cat#AM1906 GoTaq 1-Step RT-qPCR System Promega Cat#A6020 Deposited data Small RNA sequencing datasets This paper SRA: PRJNA907782 Experimental models: Cell lines Aedes albopictus C6/36 ATCC CRL-1660 Vero E6 ATCC CRL-1586 Experimental models: Organisms/strains Aedes aegypti outbred colony from Bakoumba Ref. #31 N/A Aedes aegypti Dicer2 ( / ) mutant line derived from outbred colony This study N/A Aedes aegypti Dicer2 (+/+) sister line derived from outbred colony This study N/A Oligonucleotides All oligonucleotide sequences are listed in Table S1 N/A Recombinant DNA pUb-GL3 plasmid Ref. #15 N/A (Continued on next page) 16 Cell Reports 42, 112977, August 29, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER pCMV-Rluc plasmid Ref. #15 N/A Software and algorithms FastQC http://www.bioinformatics. babraham.ac.uk/projects/ fastqc/ N/A Cutadapt Ref. #68 N/A Bowtie1 Ref. #69 N/A Rsamtools v1.20.4 (R package) Ref. #71 N/A ShortRead v1.26.0 (R package) Ref. #72 N/A Graphpad Prism v.9.3.1 www.graphpad.com N/A JMP v14.0 www.jmp.com N/A CRISPOR Ref. #62 N/A
Mutagenesis:Article Title: Multifaceted contributions of Dicer2 to arbovirus transmission by Aedes aegypti.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Dengue Virus Complex Antibody, clone D3-2H2-9-21 Merck-Milipore MAB8705; RRID: AB_95338 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Life Technologies A-11029; RRID: AB_2534088 Bacterial and virus strains DENV-1 KDH0026A Ref. #56 GenBank: HG316481 DENV-3 GA28-7 Ref. #57 GenBank: JX080299.1 ZIKV FSS13025 Ref. #58 N/A CHIKV M105 Ref. #59 GenBank: LN898104.1 MAYV TRVL 4675 Ref. #60 N/A CFAV-KPP Ref. #61 ENA: LR596014 Chemicals, peptides, and recombinant proteins Cas9 Nuclease, S. pyogenes New England Biolabs Cat#M0646 Critical commercial assays DNAzol DIRECT Molecular Research Center, Inc. Cat#DN131 TRIzol Reagent Thermo Fisher Scientific Cat#15596026 DreamTaq Green DNA Polymerase Thermo Fisher Scientific Cat#EP0714 MEGAscript T7 in vitro transcription kit Life Technologies Cat#AM1333 M-MLV Reverse Transcriptase Thermo Fisher Scientific Cat28025013 RNaseOUT Recombinant Ribonuclease Inhibitor Thermo Fisher Scientific Cat#10777019 GoTaq Probe 1-Step RT-qPCR System Promega Cat#A6002 MEGAscript RNAi kit Life Technologies Cat#AM1626M NEBNext Multiplex Small RNA Library Prep Set for Illumina New England Biolabs Cat#E7300L Universal miRNA Cloning Linker New England Biolabs Cat#S1315S Dual-Luciferase Reporter Assay System Promega Cat#E1910 MinElute PCR Purification Kit Qiagen Cat#2552893 Cellfectin II Thermo Fisher Scientific Cat#10362100 DNAseI Thermo Fisher Scientific Cat#AM1906 GoTaq 1-Step RT-qPCR System Promega Cat#A6020 Deposited data Small RNA sequencing datasets This paper SRA: PRJNA907782 Experimental models: Cell lines Aedes albopictus C6/36 ATCC CRL-1660 Vero E6 ATCC CRL-1586 Experimental models: Organisms/strains Aedes aegypti outbred colony from Bakoumba Ref. #31 N/A Aedes aegypti Dicer2 ( / ) mutant line derived from outbred colony This study N/A Aedes aegypti Dicer2 (+/+) sister line derived from outbred colony This study N/A Oligonucleotides All oligonucleotide sequences are listed in Table S1 N/A Recombinant DNA pUb-GL3 plasmid Ref. #15 N/A (Continued on next page) 16 Cell Reports 42, 112977, August 29, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER pCMV-Rluc plasmid Ref. #15 N/A Software and algorithms FastQC http://www.bioinformatics. babraham.ac.uk/projects/ fastqc/ N/A Cutadapt Ref. #68 N/A Bowtie1 Ref. #69 N/A Rsamtools v1.20.4 (R package) Ref. #71 N/A ShortRead v1.26.0 (R package) Ref. #72 N/A Graphpad Prism v.9.3.1 www.graphpad.com N/A JMP v14.0 www.jmp.com N/A CRISPOR Ref. #62 N/A
Derivative Assay:Article Title: Multifaceted contributions of Dicer2 to arbovirus transmission by Aedes aegypti.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Dengue Virus Complex Antibody, clone D3-2H2-9-21 Merck-Milipore MAB8705; RRID: AB_95338 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Life Technologies A-11029; RRID: AB_2534088 Bacterial and virus strains DENV-1 KDH0026A Ref. #56 GenBank: HG316481 DENV-3 GA28-7 Ref. #57 GenBank: JX080299.1 ZIKV FSS13025 Ref. #58 N/A CHIKV M105 Ref. #59 GenBank: LN898104.1 MAYV TRVL 4675 Ref. #60 N/A CFAV-KPP Ref. #61 ENA: LR596014 Chemicals, peptides, and recombinant proteins Cas9 Nuclease, S. pyogenes New England Biolabs Cat#M0646 Critical commercial assays DNAzol DIRECT Molecular Research Center, Inc. Cat#DN131 TRIzol Reagent Thermo Fisher Scientific Cat#15596026 DreamTaq Green DNA Polymerase Thermo Fisher Scientific Cat#EP0714 MEGAscript T7 in vitro transcription kit Life Technologies Cat#AM1333 M-MLV Reverse Transcriptase Thermo Fisher Scientific Cat28025013 RNaseOUT Recombinant Ribonuclease Inhibitor Thermo Fisher Scientific Cat#10777019 GoTaq Probe 1-Step RT-qPCR System Promega Cat#A6002 MEGAscript RNAi kit Life Technologies Cat#AM1626M NEBNext Multiplex Small RNA Library Prep Set for Illumina New England Biolabs Cat#E7300L Universal miRNA Cloning Linker New England Biolabs Cat#S1315S Dual-Luciferase Reporter Assay System Promega Cat#E1910 MinElute PCR Purification Kit Qiagen Cat#2552893 Cellfectin II Thermo Fisher Scientific Cat#10362100 DNAseI Thermo Fisher Scientific Cat#AM1906 GoTaq 1-Step RT-qPCR System Promega Cat#A6020 Deposited data Small RNA sequencing datasets This paper SRA: PRJNA907782 Experimental models: Cell lines Aedes albopictus C6/36 ATCC CRL-1660 Vero E6 ATCC CRL-1586 Experimental models: Organisms/strains Aedes aegypti outbred colony from Bakoumba Ref. #31 N/A Aedes aegypti Dicer2 ( / ) mutant line derived from outbred colony This study N/A Aedes aegypti Dicer2 (+/+) sister line derived from outbred colony This study N/A Oligonucleotides All oligonucleotide sequences are listed in Table S1 N/A Recombinant DNA pUb-GL3 plasmid Ref. #15 N/A (Continued on next page) 16 Cell Reports 42, 112977, August 29, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER pCMV-Rluc plasmid Ref. #15 N/A Software and algorithms FastQC http://www.bioinformatics. babraham.ac.uk/projects/ fastqc/ N/A Cutadapt Ref. #68 N/A Bowtie1 Ref. #69 N/A Rsamtools v1.20.4 (R package) Ref. #71 N/A ShortRead v1.26.0 (R package) Ref. #72 N/A Graphpad Prism v.9.3.1 www.graphpad.com N/A JMP v14.0 www.jmp.com N/A CRISPOR Ref. #62 N/A
Plasmid Preparation:Article Title: Multifaceted contributions of Dicer2 to arbovirus transmission by Aedes aegypti.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Dengue Virus Complex Antibody, clone D3-2H2-9-21 Merck-Milipore MAB8705; RRID: AB_95338 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Life Technologies A-11029; RRID: AB_2534088 Bacterial and virus strains DENV-1 KDH0026A Ref. #56 GenBank: HG316481 DENV-3 GA28-7 Ref. #57 GenBank: JX080299.1 ZIKV FSS13025 Ref. #58 N/A CHIKV M105 Ref. #59 GenBank: LN898104.1 MAYV TRVL 4675 Ref. #60 N/A CFAV-KPP Ref. #61 ENA: LR596014 Chemicals, peptides, and recombinant proteins Cas9 Nuclease, S. pyogenes New England Biolabs Cat#M0646 Critical commercial assays DNAzol DIRECT Molecular Research Center, Inc. Cat#DN131 TRIzol Reagent Thermo Fisher Scientific Cat#15596026 DreamTaq Green DNA Polymerase Thermo Fisher Scientific Cat#EP0714 MEGAscript T7 in vitro transcription kit Life Technologies Cat#AM1333 M-MLV Reverse Transcriptase Thermo Fisher Scientific Cat28025013 RNaseOUT Recombinant Ribonuclease Inhibitor Thermo Fisher Scientific Cat#10777019 GoTaq Probe 1-Step RT-qPCR System Promega Cat#A6002 MEGAscript RNAi kit Life Technologies Cat#AM1626M NEBNext Multiplex Small RNA Library Prep Set for Illumina New England Biolabs Cat#E7300L Universal miRNA Cloning Linker New England Biolabs Cat#S1315S Dual-Luciferase Reporter Assay System Promega Cat#E1910 MinElute PCR Purification Kit Qiagen Cat#2552893 Cellfectin II Thermo Fisher Scientific Cat#10362100 DNAseI Thermo Fisher Scientific Cat#AM1906 GoTaq 1-Step RT-qPCR System Promega Cat#A6020 Deposited data Small RNA sequencing datasets This paper SRA: PRJNA907782 Experimental models: Cell lines Aedes albopictus C6/36 ATCC CRL-1660 Vero E6 ATCC CRL-1586 Experimental models: Organisms/strains Aedes aegypti outbred colony from Bakoumba Ref. #31 N/A Aedes aegypti Dicer2 ( / ) mutant line derived from outbred colony This study N/A Aedes aegypti Dicer2 (+/+) sister line derived from outbred colony This study N/A Oligonucleotides All oligonucleotide sequences are listed in Table S1 N/A Recombinant DNA pUb-GL3 plasmid Ref. #15 N/A (Continued on next page) 16 Cell Reports 42, 112977, August 29, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER pCMV-Rluc plasmid Ref. #15 N/A Software and algorithms FastQC http://www.bioinformatics. babraham.ac.uk/projects/ fastqc/ N/A Cutadapt Ref. #68 N/A Bowtie1 Ref. #69 N/A Rsamtools v1.20.4 (R package) Ref. #71 N/A ShortRead v1.26.0 (R package) Ref. #72 N/A Graphpad Prism v.9.3.1 www.graphpad.com N/A JMP v14.0 www.jmp.com N/A CRISPOR Ref. #62 N/A
other:Article Title: University population-based prospective cohort study of SARS-CoV-2 infection and immunity (SARSSURV-ULiège): a study protocol
Article Snippet: 382 383 Quantitative reverse-transcriptase polymerase chain reaction (RT-qPCR) assay 384 We perform a multiplex RT-qPCR assay using the TaqPath RT-PCR COVID-19 kit (Thermo Fisher A47817) 385 together with the TaqPath 1-step master mix – No ROX (Thermo Fisher CN A28523).
Article Title: Relationship between microRNA expression profiling and gestational diabetes: A systematic review
Article Snippet: RT-qPCR U6 n/a n/a HUANG et al., 2021* Trizol n/a RT-qPCR GAPDH n/a n/a Li 2015ɨ** RecoverAllTM Total Nucleic Acid Isolation kit (Ambion) y TRNzol reagent (TIANGEN Biotech, Beijing, China) miRNA first-strand cDNA kit (TIANGEN Biotech, Beijing, China) miRcute miRNA qPCR detection kit (TIANGEN Biotech, Beijing, China) U6 Agilent Human miRNA Microarray (Agilent) n/a Li et al., 2018** KingFisher Automated Purification Kits (Thermo Fisher) miRCURY LNATM microRNA Array Hi-Power Labeling kit (Qiagen) n/a Hairpin-itTMmiRNAs RealTime PCR Quantization Kit (GenePharma) n/a n/a n/a Li W 2022* TRIzol reagent (Magen Biotechnology Co.) TaKaRa PrimeScript II 1st Strand cDNA Synthesis Kit (TaKaRa) miScript SYBR Green PCR Kit (TaKaRa) U6 n/a n/a Peng et al., 2019* Trizol (Ambion, USA) miRcute miRNA FirstStrand cDNA Synthesis Kit (TianGen, Beijing, China) miRcute miRNA qPCR Detection Kit (SYBR Green) (TianGen, Beijing China) U6 n/a n/a Sun et al., 2020* Trizol reagent (Invitrogen, Carlsbad, CA, USA) TaqMan MicroRNA Reverse Transcription Kit TaqMan Univel PCR Master Mix (Applied Biosystems, Foster City, CA, USA) U6 n/a n/a Tang 2020ᵻ* Trizol Reagent (Invitrogen, Carlsbad, CA, USA) M-MLV Reverse Transcriptase (Invitrogen) qRT–PCR (BIONEER, Daejeon, Korea) U6 n/a NEBNext Multiplex Small RNA Library Prep Set for Illumina_x0003_ (NEB, Ipswich, MA, USA) Wang S 2020* Trizol reagent (Invitrogen) Transcriptase Kit (Takara, Otsu, Japan) SYBR Green PCR Master Mix (Takara) U6 n/a n/a Zhang Lu 2021* Trizol n/a RT-qPCR U6 n/a n/a Zhao et al., 2014* Trizol reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) TaqMan MicroRNA Reverse Transcription kit TaqMan microRNA assay (Applied Biosystems, Branchburg, NJ, USA) U6 n/a n/a Serum Abdeltawab et al., 2020** miRVana PARIS kits (Life Technologies, Grand Island, NY, USA) TaqMan MicroRNA Reverse Transcription Kit TaqMan quantitative RTPCR.
TLDA Assay:Article Title: miRNA-34b as a tumor suppressor in estrogen-dependent growth of breast cancer cells
Article Snippet: The number of colonies was counted after they were stained with 0.05% crystal violet (Sigma-Aldrich) for 1 hour and washed extensively with PBS. .. The human miRNA TLDA was used in conjunction with Multiplex reverse transcriptase (RT; Applied Biosystems) consisting of eight predefined RT primer pools containing up to 48 RT primers each. ..
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